MICROBIAL GENERATION OF SILVER NANOPARTICLES BY SYNTHESIZING ENTEROCOCCUS FAECALIS STRAIN MI103

Jane NSOFOR, Imade Yolanda NSA, Busayo Tosin AKINYEMI, Adewale Kayode OGUNYEMI, Matthew Olusoji ILORI

Abstract

Silver nanoparticles (AgNPs) have recently attracted attention for their potential as a source of new antimicrobials, with the potential to better fight a wide range of pathogenic bacteria. Nanomaterials are now commonly used for the production of industrial and domestic items.  As a result of increase in the menace and threats to live by antibiotic-resistant bacteria, nanoparticles have been proposed as alternative for its control. Enterococcus faecalis, strain MI103, was selected out of four isolates obtained from a soil sample and identified using 16S rRNA gene sequencing method. The selected bacterial isolate synthesized AgNPs by reduction of Silver nitrate (AgNO3) solution (incubated for 2 days at room temperature). Colour development from pale yellow to brown was observed which indicated the extracellular production of AgNPs.  AgNPs was further characterized by UV/VIS spectroscopy, Scanning Electron Microscopy (JOEL-JSM-7660F) (shape) and Energy Dispersive X-ray (EDX) (composition).  The synthesized AgNPs ranged in size of 90-100 nm and were of cylindrical shape. It was found that the highest quantity of AgNO3 was produced at pH 7. The synthesized AgNPs had strong bactericidal activity against MRSA. 

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