RAT KIDNEY TISSUES: OXIDATIVE MODIFICATION OF PROTEINS UNDER WATER AND SALT LOADS ASSOCIATED WITH HgCl2 NEPHROPATHY

Abstract

Some changes in the main indexes of the pro-oxidation system of rat’s kidney tissues exposed to water and salt load on background of the nephropathy caused by mercury chloride were investigated. Conditions of the free-radical oxidation of kidney tissues’ proteins under water and salt load alone or complicated by the nephropathy have been analyzed. It is found that water and salt load leads to a higher content of the thiobarbiturate reaction products (TBA-RP) in kidney tissues in comparison with the control experiments while the content of the protein oxidative modification products remains unchanged. Thus, water and salt load causes a higher activation level of free radical oxidation of lipids. A histochemical investigation has been performed to evaluate a degree of the oxidative modification of proteins in rat kidney tissues under water and salt load complicated by the mercuric nephropathy and a higher level of the oxidative modification of proteins has been determined by the Red/Blue coefficient in rat kidneys irrespectively of the load type in case of intoxication with mercury chloride.