UNIVERSAL FLUOROMETER BASED ON FLUORESCENCE QUENCHING

Abstract

This paper describes a method and portable device meant to determine the concentration of  various  chemical  species  in  solutions  based  on  the fluorescence  quenching phenomenon.  The solution brought about includes the possibility of measuring the concentration of more chemical or biological species using the same optoelectronic fluorescent, requiring    the change of a fluorofor capsule only when going from a chemical species to another and setting of the chemical species type analyzed.  In addition to the quantitative analysis made by means of the apparatus described, assisted  by  computer  and  specialized  software,  it  is  possible  to  carry  out  a  spectral  scanning
fluorescence in all spectral range covered by monochromatic radiation sources of LED type of the device  in  question.  It  allows  also  carrying  out  simultaneously  both  qualitative  and  semi-quantitative analysis of all fluorescent species in the solution tested.