EVALUATION OF THE TOXICOLOGICAL SAFETY OF A FREEZE- DRIED PROBIOTIC PREPARATION

Zapryana DENKOVA, Ivan MURGOV, Rositsa DENKOVA, Rositsa ENIKOVA

Abstract

A combination consisting of selected probiotic strains lactobacilli and bifidobacteria from the  National  Bank  for  Industrial  Microorganisms  and  Cell  Cultures  (NBIMCC),  Bulgaria  was developed.  During  their  joint  cultivation  the  strains demonstrated  their  ability  to  multiplicate, accumulating high concentrations of viable cells. The  toxicogenity  of  the  symbiotic  combination  was  examined  by  testing  the  freeze-dryed  substance, containing  lactobacilli  and  bifidobacteria,  on  biological  objects  (white  mice).  Each experimental animal was injected intraperitoneally with 5.108 CFU viable cells daily in the duration of 10 days. After the experimental period a post-mortem examination was conducted to analyze the safety of the substance  of  probiotic  lactobacilli  and  bifidobacteria,  which  is  the  basis  for  the  preparation  of
probiotic  combinations.  The  absence  of  toxicogenity  of  the  freeze-dried  substance  of  probiotic lactobacilli and bifidobacteria is proven by the lack of any pathological changes in the health status, damages  to  the  parenchymal  organs,  the  peritoneum or  the  omentum that  would  otherwise  indicate toxicity.

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