GENETIC, BIOCHEMICAL AND PHYSIOLOGICAL CHARACTERISTICS OF THE RECOMBINANT STRAIN LACTOBACILLUS RL15, OBTAINED BY INTERGENERIC HYBRIDIZATION BETWEEN LACTOBACILLUS ACIDOPHILUS 2 AND BIFIDOBACTERIUM BIFIDUM L1

Rositsa DENKOVA, I DOBREV, Zapriana DENKOVA, Velichka YANAKIEVA, Zoltan URSHEV, Maria YORDANOVA, Svetla ILIEVA

Abstract

A successful intergeneric recombination by protoplast fusion between Lactobacillus acidophilus 2 and Bifidobacterium bifidum L1 is performed. The strain Lactobacillus RL15 with high reproductive capacity and moderate acidity, that doesn’t cause sliming of milk is selected. Using modern molecular  genetic methods it has been found that the hybrid inherits the genome of one of the parental strains. It is shown that in the united genome occur changes, which are expressed in the hybrid’s phenotypic properties, enzyme profile and technological parameters.

 

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