MODIFIED PRUSSIAN BLUE SCREEN PRINTED ELECTRODES FOR H2O2 DETECTION

Florentina HUTANU, Gheorghe GUTT

Abstract

In this work, presents recent developments in the electrochemical application of disposable screen-printed sensors, to the type of materials used to modify the working electrode.The sensor was based on the electrocatalytic reduction of H2O2 on Prussian Blue modified screen-printed electrode. A comparative study regarding different procedures for modifying the carbon screen-printed electrodes (SPE) with Prussian Blue (PB) was carried out in this work. Two procedures for PB deposition on the SPE electrodes were tested: electrochemical deposition (galvanostatic, cyclic voltammetry) and chemical deposition by the reaction of K3[Fe(CN)6] with FeCl3.Also, the influence of the pretreatment of SPE (+1.7V for 3 min in PBS, pH 7.4) and of the stabilization of PB deposited on SPE (by heating at 100°C) were evaluated. The developed sensors were optimized with respect to the lowest limit of detection achieved for amperometric detection of H2O2. Analytical parameters, such as detection limit, linearity range and sensitivity have been evaluated, together with operational and storage stability. The improved electro-deposition methods, pH stability and permeability of the optimized PB film provide a further boost in its sensitivity for H2O2 detection, which is a critical parameter in biosensor design and application.

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