SEPARATION AND QUANTIFICATION OF BIOGENIC AMINES IN BANANAS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

This paper reports separation and quantification of biogenic amines in bananas by high performance liquid chromatography. Fresh and preserved samples (seven days of storage at refrigeration temperature, at room temperature respectively) were analyzed. We obtained the separation of all nine biogenic amines (tryptamine, 2-phenylethylamine, putrescine, cadaverine, histamine, serotonin, tyramine, spermidine, spermine) with good resolution, including good resolution of serotonin and internal standard (1,7diaminoheptane). Mean biogenic amines levels of 10.344 mg/kg (tryptamine) to 61.502 mg/kg (putrescine) were found in fresh bananas. After seven days of storage at room temperature, 2-phenylethylamine decreases significantly. After seven days of storage at refrigeration temperature, tryptamine increases significantly, whereas phenylethylamine decreases significantly.